1: Appl Biochem Biotechnol. 2006
Nov;135(2):133-44.Links...
Electrolyzed-reduced (hydrogen rich) water protects
against oxidative damage to DNA, RNA, and protein. Lee
MY, Kim YK, Ryoo KK, Lee YB, Park EJ.
Department of Genetic Engineering, Soonchunhyang
University, Asan, Chungnam 336-600, Korea.
miyoung@sch.ac.kr
The generation of reactive oxygen species is thought
to cause extensive oxidative damage to various
biomolecules such as DNA, RNA, and protein.
In this study, the preventive, suppressive, and
protective effects of in vitro supplementation with
electrolyzed-reduced water on H2O2-induced DNA damage
in human lymphocytes were examined using a comet
assay. Pretreatment, cotreatment, and posttreatment
with electrolyzed-reduced water enhanced human
lymphocyte resistance to the DNA strand breaks induced
by H2O2 in vitro.
Moreover, electrolyzed-reduced water was much more
effective than diethylpyrocarbonate-treated water in
preventing total RNA degradation at 4 and 25 degrees
C. In addition, electrolyzed-reduced water completely
prevented the oxidative cleavage of horseradish
peroxidase, as determined using sodium dodecyl
sulfate-polyacrylamide gels.
Enhancement of the antioxidant activity of ascorbic
acid dissolved in electrolyzed-reduced water was about
threefold that of ascorbic acid dissolved in
nonelectrolyzed deionized water, as measured by a
xanthine-xanthine oxidase superoxide scavenging assay
system, suggesting an inhibitory effect of
electrolyzedreduced water on the oxidation of ascorbic
acid.
PMID: 17159237 [PubMed - indexed for MEDLINE]
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